NASC

NASC ID: N849761

Name: WiscDsLox253D04

ABRC stock number: CS849761

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox253D04.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G61880.2 (chr 3 pos 22906069 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796725.

Wiscseq_DsLox253D04.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G20820.1 (chr 3 pos 7280893 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796724.

Wiscseq_DsLox253D04.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G23630.1-UTR3 and AT3G23633.1-UTR3 (at chr 3 pos 8495801 (C/8495801-8495879) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 0, R 7, C 4 N 0. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453295.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox253D04

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		CYP78A9	AT3G23630 AT3G23633 At3g61880

EMBL accession:

CW793307